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An electrochemical aptasensor for detection of bovine interferon gamma

Abstract

Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-γ) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-γ. A thiolated IFN-γ-binding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by self-assembly. Binding of IFN-γ to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN- with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for pan-side testing of bovine blood to help better identify and contain breakouts of bovine TB.

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Publication details

The article was received on 26 May 2017, accepted on 10 Jul 2017 and first published on 17 Jul 2017


Article type: Paper
DOI: 10.1039/C7AY01313B
Citation: Anal. Methods, 2017, Accepted Manuscript
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    An electrochemical aptasensor for detection of bovine interferon gamma

    B. P. Crulhas, D. J. Hadley, Y. Liu, D. Shin, G. Stybayeva, M. Imanbekova, A. E. Hill, V. A. Pedrosa and A. Revzin, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01313B

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