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Issue 5, 2016
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Gender specific differences in the liver proteome of rats exposed to short term and low-concentration hexabromocyclododecane (HBCD)

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Abstract

The influence of short term (7-day) exposure of male rats to the brominated flame retardant hexabromocyclododecane (HBCD) was studied by investigation of the liver proteome, both in euthyroid and hypothyroid rats and by comparing results with general data on animal physiology and thyroid hormone, leptin, insulin and gonadotropin concentrations determined in parallel. Proteome analysis of liver tissue by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) revealed that only small protein pattern changes were induced by exposure in males, on just a few proteins with different functions and not involved in pathways in common. This is in contrast to previous findings in similarly exposed eu- and hypothyroid female rats, where general metabolic pathways had been shown to be affected. The largest gender-dependent effects concerned basal concentrations of liver proteins already in control and hypothyroid animals, involving mainly the pathways which were also differently affected by HBCD exposure. Among them were differences in lipid metabolism, which – upon exposure to HBCD – may also be the reason for the considerably higher ratio of γ-HBCD accumulated in white adipose tissue of exposed female rats compared to males. The results further elucidate the already suggested different sensitivity of genders towards HBCD exposure on the protein level, and confirm the need for undertaking toxicological animal experiments in both genders.

Graphical abstract: Gender specific differences in the liver proteome of rats exposed to short term and low-concentration hexabromocyclododecane (HBCD)

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Publication details

The article was received on 26 Apr 2016, accepted on 23 Jun 2016 and first published on 30 Jun 2016


Article type: Paper
DOI: 10.1039/C6TX00166A
Citation: Toxicol. Res., 2016,5, 1273-1283
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    Gender specific differences in the liver proteome of rats exposed to short term and low-concentration hexabromocyclododecane (HBCD)

    I. Miller, C. Diepenbroek, E. Rijntjes, J. Renaut, K. J. Teerds, C. Kwadijk, S. Cambier, A. J. Murk, A. C. Gutleb and T. Serchi, Toxicol. Res., 2016, 5, 1273
    DOI: 10.1039/C6TX00166A

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