Jump to main content
Jump to site search

Issue 14, 2016
Previous Article Next Article

Using membrane composition to fine-tune the pKa of an optical liposome pH sensor

Author affiliations

Abstract

Liposomes containing membrane-anchored pH-sensitive optical probes are valuable sensors for monitoring pH in various biomedical samples. The sensitivity of the sensor is maximized when the probe pKa is close to the expected sample pH. While some biomedical samples are close to neutral pH there are several circumstances where the pH is 1 or 2 units lower. Thus, there is a need to fine-tune the probe pKa in a predictable way. This investigation examined two lipid-conjugated optical probes, each with appended deep-red cyanine dyes containing indoline nitrogen atoms that are protonated in acid. The presence of anionic phospholipids in the liposomes stabilized the protonated probes and increased the probe pKa values by <1 unit. The results show that rational modification of the membrane composition is a general non-covalent way to fine-tune the pKa of an optical liposome sensor for optimal pH sensing performance.

Graphical abstract: Using membrane composition to fine-tune the pKa of an optical liposome pH sensor

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Oct 2015, accepted on 01 Dec 2015 and first published on 01 Dec 2015


Article type: Paper
DOI: 10.1039/C5TC03480A
Citation: J. Mater. Chem. C, 2016,4, 2925-2930
  •   Request permissions

    Using membrane composition to fine-tune the pKa of an optical liposome pH sensor

    K. J. Clear, K. Virga, L. Gray and B. D. Smith, J. Mater. Chem. C, 2016, 4, 2925
    DOI: 10.1039/C5TC03480A

Search articles by author

Spotlight

Advertisements