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Issue 14, 2016
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Docking-guided identification of protein hosts for GFP chromophore-like ligands

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Abstract

Synthetic analogs of the Green Fluorescent Protein (GFP) chromophore emerge as promising fluorogenic dyes for labeling in living systems. Here, we report the computational identification of protein hosts capable of binding to and enhancing fluorescence of GFP chromophore derivatives. Automated docking of GFP-like chromophores to over 3000 crystal structures of Escherichia coli proteins available in the Protein Data Bank allowed the identification of a set of candidate proteins. Four of these proteins were tested experimentally in vitro for binding with the GFP chromophore and its red-shifted Kaede chromophore-like analogs. Two proteins were found to possess sub-micromolar affinity for some Kaede-like chromophores and activate fluorescence of these fluorogens.

Graphical abstract: Docking-guided identification of protein hosts for GFP chromophore-like ligands

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Publication details

The article was received on 23 Nov 2015, accepted on 23 Feb 2016 and first published on 23 Feb 2016


Article type: Paper
DOI: 10.1039/C5TC03931B
Citation: J. Mater. Chem. C, 2016,4, 3036-3040
  • Open access: Creative Commons BY license
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    Docking-guided identification of protein hosts for GFP chromophore-like ligands

    N. V. Povarova, N. G. Bozhanova, K. S. Sarkisyan, R. Gritcenko, M. S. Baranov, I. V. Yampolsky, K. A. Lukyanov and A. S. Mishin, J. Mater. Chem. C, 2016, 4, 3036
    DOI: 10.1039/C5TC03931B

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