Jump to main content
Jump to site search

Issue 1, 2016
Previous Article Next Article

Ammonia sensing for enzymatic urea detection using organic field effect transistors and a semipermeable membrane

Author affiliations

Abstract

Organic Field Effect Transistors (OFETs) are used to measure ammonia in solution via ammonia diffusion into the OFET channel. An increase in ammonia concentrations results in a decrease in transistor currents. The regeneration of the OFET current after ammonia uptake is slow, which allows us to read out the maximum ammonia dose which was applied. A 100 nm parylene-C layer serves as a semipermeable top gate dielectric. The parylene layer is functionalized with the covalently attached enzyme urease. The enzyme catalyses the hydrolysis of urea to ammonia and carbon dioxide, i.e. urea can be detected via its hydrolysis product ammonia. The sensitivity covers a range of physiological concentrations of urea, which are several mM.

Graphical abstract: Ammonia sensing for enzymatic urea detection using organic field effect transistors and a semipermeable membrane

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Sep 2015, accepted on 17 Nov 2015 and first published on 17 Nov 2015


Article type: Paper
DOI: 10.1039/C5TB02025E
Citation: J. Mater. Chem. B, 2016,4, 162-168
  • Open access: Creative Commons BY license
  •   Request permissions

    Ammonia sensing for enzymatic urea detection using organic field effect transistors and a semipermeable membrane

    F. X. Werkmeister, T. Koide and B. A. Nickel, J. Mater. Chem. B, 2016, 4, 162
    DOI: 10.1039/C5TB02025E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements