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Issue 62, 2016, Issue in Progress
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Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii

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Abstract

Cephalopods have recently emerged as a source of inspiration for the development of novel functional materials. Within this context, a number of studies have explored structural proteins known as reflectins, which play a key role in cephalopod adaptive coloration in vivo and exhibit interesting properties in vitro. Herein, we report an improved high-yield strategy for the preparation and isolation of reflectins in quantities sufficient for materials applications. We first select the Doryteuthis (Loligo) pealeii reflectin A2 (RfA2) isoform as a “model” system and validate our approach for the expression and purification of this protein. We in turn fabricate RfA2-based two-terminal devices and employ both direct and alternating current measurements to demonstrate that RfA2 films conduct protons. Our findings underscore the potential of reflectins as functional materials and may allow a wider range of researchers to investigate their properties.

Graphical abstract: Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii

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Publication details

The article was received on 29 Feb 2016, accepted on 28 May 2016 and first published on 15 Jun 2016


Article type: Communication
DOI: 10.1039/C6RA05405F
Citation: RSC Adv., 2016,6, 57103-57107
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    Production and electrical characterization of the reflectin A2 isoform from Doryteuthis (Loligo) pealeii

    D. D. Ordinario, L. Phan, W. G. Walkup IV, Y. Van Dyke, E. M. Leung, M. Nguyen, A. G. Smith, J. Kerr, M. Naeim, I. Kymissis and A. A. Gorodetsky, RSC Adv., 2016, 6, 57103
    DOI: 10.1039/C6RA05405F

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