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Issue 46, 2016, Issue in Progress
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Monodisperse magnetic mesoporous silica microspheres facilitate the studies of gastric cancer-specific peptides in sera

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Abstract

Background: The prognosis of gastric cancer remains poor despite the recent improvements in therapies. The aim of this study was to develop a novel strategy to discover gastric cancer-specific peptides in sera. Methods: Here, Fe3O4@mSiO2 microspheres were prepared to separate and enrich serum peptides in patients with gastric cancer. Stable isotope labeling coupled with LC-MALDI then quantified those differentially expressed peptides. In contrast with the commonly used strategy, this novel strategy was more efficient and sensitive, which also enabled the accurate identification and quantification of serum peptides. Results: Based on this strategy, the results showed that serum CK18, apoE, C3 precursor, Clusterin, C4a, HMW-kininogen, apoA-4 and ghrelin peptides were differentially expressed significantly in patients, especially when there had been no available information concerning the serum level of active ghrelin in predicting gastric cancer. In addition, our data demonstrated that serum active ghrelin expression was closely correlated with shorter survival in patients which would show biological activity for promotion of lymph node metastasis. Conclusions: Active ghrelin might be a novel circulating biomarker in the prognosis of gastric cancer.

Graphical abstract: Monodisperse magnetic mesoporous silica microspheres facilitate the studies of gastric cancer-specific peptides in sera

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Publication details

The article was received on 21 Dec 2015, accepted on 14 Apr 2016 and first published on 18 Apr 2016


Article type: Paper
DOI: 10.1039/C5RA27378A
Citation: RSC Adv., 2016,6, 39963-39971
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    Monodisperse magnetic mesoporous silica microspheres facilitate the studies of gastric cancer-specific peptides in sera

    C. Meng, Z. Zhen-Xian, C. Peng, W. Kun, Z. Gang-Tian, F. Yu-Qi, Y. Shi-Hai, H. Cheng-Cai, G. Zhi-Rong, M. Xiao-Dong, Z. Ning-Wei and L. Chao, RSC Adv., 2016, 6, 39963
    DOI: 10.1039/C5RA27378A

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