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Issue 8, 2016
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Cellulose as an efficient matrix for lipase and transaminase immobilization

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Abstract

Immobilization of enzymes is important to improve their stability and to facilitate their recyclability, aiming to make biocatalytic processes more efficient. One of the important aspects is the utilization of cheap, abundant, and environmentally friendly carriers for enzyme immobilization. Here we report the use of functionalized cellulose for lipase and transaminase immobilization. High immobilization efficiencies (up to 90%) could be achieved for the transaminase from Vibrio fluvialis. For immobilized lipase CAL-B as well as the transaminase, good conversions and recyclability could be demonstrated in kinetic resolutions to afford chiral alcohols or amines. Moreover, such application of the immobilized transaminase enabled very high conversions in a continuous-flow process in the asymmetric synthesis of (S)-phenylethylamine (80% conversion, >99% ee).

Graphical abstract: Cellulose as an efficient matrix for lipase and transaminase immobilization

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Publication details

The article was received on 24 Nov 2015, accepted on 14 Dec 2015 and first published on 17 Dec 2015


Article type: Paper
DOI: 10.1039/C5RA24976G
Author version available: Download Author version (PDF)
Citation: RSC Adv., 2016,6, 6665-6671
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    Cellulose as an efficient matrix for lipase and transaminase immobilization

    S. P. de Souza, I. I. Junior, G. M. A. Silva, L. S. M. Miranda, M. F. Santiago, F. Leung-Yuk Lam, A. Dawood, U. T. Bornscheuer and R. O. M. A. de Souza, RSC Adv., 2016, 6, 6665
    DOI: 10.1039/C5RA24976G

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