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Issue 2, 2016
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Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability

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Abstract

Bright, long-lasting and non-phototoxic organic fluorophores are essential to the continued advancement of biological imaging. Traditional approaches towards achieving photostability, such as the removal of molecular oxygen and the use of small-molecule additives in solution, suffer from potentially toxic side effects, particularly in the context of living cells. The direct conjugation of small-molecule triplet state quenchers, such as cyclooctatetraene (COT), to organic fluorophores has the potential to bypass these issues by restoring reactive fluorophore triplet states to the ground state through intra-molecular triplet energy transfer. Such methods have enabled marked improvement in cyanine fluorophore photostability spanning the visible spectrum. However, the generality of this strategy to chemically and structurally diverse fluorophore species has yet to be examined. Here, we show that the proximal linkage of COT increases the photon yield of a diverse range of organic fluorophores widely used in biological imaging applications, demonstrating that the intra-molecular triplet energy transfer mechanism is a potentially general approach for improving organic fluorophore performance and photostability.

Graphical abstract: Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability

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Publication details

The article was received on 27 Oct 2015, accepted on 17 Dec 2015 and first published on 18 Dec 2015


Article type: Paper
DOI: 10.1039/C5PP00400D
Author version available: Download Author version (PDF)
Citation: Photochem. Photobiol. Sci., 2016,15, 196-203
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    Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability

    Q. Zheng, S. Jockusch, G. G. Rodríguez-Calero, Z. Zhou, H. Zhao, R. B. Altman, H. D. Abruña and S. C. Blanchard, Photochem. Photobiol. Sci., 2016, 15, 196
    DOI: 10.1039/C5PP00400D

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