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Issue 9, 2016
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Hybrid phase ligation for efficient synthesis of histone proteins

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Abstract

We introduce a hybrid solid-solution phase ligation approach that combines the efficiency of solid phase ligation with solution phase ligation in the total synthesis of modified histone proteins. A two linker strategy allows analysis throughout work on the solid phase and maximizes yields through cleavage at an external Rink, while an internal HMBA linker allows the native carboxyl terminus for any protein sequence. We demonstrate this approach for two histone proteins: triple-acetylated H4-K5ac, K12ac, K91ac and CENP-A-K124ac.

Graphical abstract: Hybrid phase ligation for efficient synthesis of histone proteins

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Publication details

The article was received on 23 Oct 2015, accepted on 15 Jan 2016 and first published on 20 Jan 2016


Article type: Communication
DOI: 10.1039/C5OB02195B
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2016,14, 2603-2607
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    Hybrid phase ligation for efficient synthesis of histone proteins

    R. R. Yu, S. K. Mahto, K. Justus, M. M. Alexander, C. J. Howard and J. J. Ottesen, Org. Biomol. Chem., 2016, 14, 2603
    DOI: 10.1039/C5OB02195B

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