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Issue 26, 2016
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Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase

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Abstract

SalL, an enzyme that catalyzes the synthesis of SAM from L-methionine and 5′-chloro-5′-deoxyoadenosine, is shown to accept 5′-chloro-5′-deoxythienoadenosine as a substrate and facilitate the synthesis of a synthetic SAM analog with an unnatural nucleobase. This synthetic cofactor is demonstrated to replace SAM in the DNA methylation reaction with M.TaqI.

Graphical abstract: Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase

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Publication details

The article was received on 19 Apr 2016, accepted on 24 May 2016 and first published on 24 May 2016


Article type: Communication
DOI: 10.1039/C6OB00844E
Citation: Org. Biomol. Chem., 2016,14, 6189-6192
  • Open access: Creative Commons BY-NC license
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    Chemoenzymatic synthesis and utilization of a SAM analog with an isomorphic nucleobase

    C. Vranken, A. Fin, P. Tufar, J. Hofkens, M. D. Burkart and Y. Tor, Org. Biomol. Chem., 2016, 14, 6189
    DOI: 10.1039/C6OB00844E

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