Issue 17, 2016
From the journal:

Organic & Biomolecular Chemistry

Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans†

Angie D. Calderon,a   Yunpeng Liu,ab   Xu Li,a   Xuan Wang,a   Xi Chen,c   Lei Li*a  and  Peng G. Wang*a  

* Corresponding authors

a Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA
E-mail: lli22@gsu.edu, pwang11@gsu.edu

b Chemily LLC, Atlanta, GA 30303, USA

c Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA

Abstract

Substrate specificity studies of human FUT8 using 77 structurally-defined N-glycans as acceptors showed a strict requirement towards the α1,3-mannose branch, but a great promiscuity towards the α1,6-mannose branch. Accordingly, a chemoenzymatic strategy was developed for the efficient synthesis of core-fucosylated asymmetric N-glycans.

Graphical abstract: Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans

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Article information

DOI
https://doi.org/10.1039/C6OB00586A
Article type
Communication
Submitted
17 Mar 2016
Accepted
01 Apr 2016
First published
04 Apr 2016

Org. Biomol. Chem., 2016,14, 4027-4031

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Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans

A. D. Calderon, Y. Liu, X. Li, X. Wang, X. Chen, L. Li and P. G. Wang, Org. Biomol. Chem., 2016, 14, 4027 DOI: 10.1039/C6OB00586A

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