Jump to main content
Jump to site search

Issue 7, 2016
Previous Article Next Article

An improved fluorogenic assay for SIRT1, SIRT2, and SIRT3

Author affiliations

Abstract

Sirtuins are NAD-dependent lysine deacylases that play critical roles in cellular regulation and are implicated in human diseases. Modulators of sirtuins are needed as tools for investigating their biological functions and possible therapeutic applications. However, the discovery of sirtuin modulators is hampered by the lack of efficient sirtuin assays. Here we report an improved fluorogenic assay for SIRT1, SIRT2, and SIRT3 using a new substrate, a myristoyl peptide with a C-terminal aminocoumarin. The new assay has several advantages, including significantly lower substrate concentration needed, increased signal-to-background ratio, and improved Z′-factor. The novel assay thus will expedite high-throughput screening of SIRT1, SIRT2, and SIRT3 modulators.

Graphical abstract: An improved fluorogenic assay for SIRT1, SIRT2, and SIRT3

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Dec 2015, accepted on 12 Jan 2016 and first published on 12 Jan 2016


Article type: Communication
DOI: 10.1039/C5OB02609A
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2016,14, 2186-2190
  •   Request permissions

    An improved fluorogenic assay for SIRT1, SIRT2, and SIRT3

    Y. Chiang and H. Lin, Org. Biomol. Chem., 2016, 14, 2186
    DOI: 10.1039/C5OB02609A

Search articles by author

Spotlight

Advertisements