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Issue 1, 2017
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Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging

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Abstract

Ultrasound is critical in many areas of medicine including obstetrics, oncology, and cardiology with emerging applications in regenerative medicine. However, one critical limitation of ultrasound is the low contrast of target tissue over background. Here, we describe a novel cup-shaped silica nanoparticle that is reminiscent of exosomes and that has significant ultrasound impedance mismatch for labelling stem cells for regenerative medicine imaging. These exosome-like silica nanoparticles (ELS) were created through emulsion templating and the silica precursors bis(triethoxysilyl)ethane (BTSE) and bis(3-trimethoxysilyl-propyl)amine (TSPA). We found that 40% TSPA resulted in the exosome like-morphology and a positive charge suitable for labelling mesenchymal stem cells. We then compared this novel structure to other silica structures used in ultrasound including Stober silica nanoparticles (SSN), MCM-41 mesoporous silica nanoparticles (MSN), and mesocellular foam silica nanoparticles (MCF) and found that the ELS offered enhanced stem cell signal due to its positive charge to facilitate cell uptake as well as inherently increased echogenicity. The in vivo detection limits were <500 cells with no detectable toxicity at the concentrations used for labelling. This novel structure may eventually find utility in applications beyond imaging requiring an exosome-like shape including drug delivery.

Graphical abstract: Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging

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Publication details

The article was received on 19 Oct 2016, accepted on 29 Nov 2016 and first published on 01 Dec 2016


Article type: Paper
DOI: 10.1039/C6NR08177K
Citation: Nanoscale, 2017,9, 402-411
  • Open access: Creative Commons BY license
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    Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging

    F. Chen, M. Ma, J. Wang, F. Wang, S. Chern, E. R. Zhao, A. Jhunjhunwala, S. Darmadi, H. Chen and J. V. Jokerst, Nanoscale, 2017, 9, 402
    DOI: 10.1039/C6NR08177K

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