Issue 33, 2016

Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

Abstract

The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling. We report the use of cantilever arrays for simultaneous detection of multiple exosomal surface-antigens with high sensitivity and selectivity. Exosomes from breast cancer were selectively identified by detecting over-expressed membrane-proteins CD24, CD63, and EGFR. Excellent selectivity however, was achieved when targeting the cell-surface proteoglycan, Glypican-1 at extraordinary limits (∼200 exosomes per mL, ∼0.1 pg mL−1).

Graphical abstract: Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

Supplementary files

Article information

Article type
Communication
Submitted
29 Apr 2016
Accepted
27 Jul 2016
First published
28 Jul 2016

Nanoscale, 2016,8, 15137-15141

Author version available

Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes

H. Etayash, A. R. McGee, K. Kaur and T. Thundat, Nanoscale, 2016, 8, 15137 DOI: 10.1039/C6NR03478K

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