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Issue 23, 2016
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Analysing intracellular deformation of polymer capsules using structured illumination microscopy

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Abstract

Understanding the behaviour of therapeutic carriers is important in elucidating their mechanism of action and how they are processed inside cells. Herein we examine the intracellular deformation of layer-by-layer assembled polymer capsules using super-resolution structured illumination microscopy (SIM). Spherical- and cylindrical-shaped capsules were studied in three different cell lines, namely HeLa (human epithelial cell line), RAW264.7 (mouse macrophage cell line) and differentiated THP-1 (human monocyte-derived macrophage cell line). We observed that the deformation of capsules was dependent on cell line, but independent of capsule shape. This suggests that the mechanical forces, which induce capsule deformation during cell uptake, vary between cell lines, indicating that the capsules are exposed to higher mechanical forces in HeLa cells, followed by RAW264.7 and then differentiated THP-1 cells. Our study demonstrates the use of super-resolution SIM in analysing intracellular capsule deformation, offering important insights into the cellular processing of drug carriers in cells and providing fundamental knowledge of intracellular mechanobiology. Furthermore, this study may aid in the design of novel drug carriers that are sensitive to deformation for enhanced drug release properties.

Graphical abstract: Analysing intracellular deformation of polymer capsules using structured illumination microscopy

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Publication details

The article was received on 15 Mar 2016, accepted on 16 May 2016 and first published on 31 May 2016


Article type: Paper
DOI: 10.1039/C6NR02151D
Citation: Nanoscale, 2016,8, 11924-11931
  • Open access: Creative Commons BY license
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    Analysing intracellular deformation of polymer capsules using structured illumination microscopy

    X. Chen, J. Cui, H. Sun, M. Müllner, Y. Yan, K. F. Noi, Y. Ping and F. Caruso, Nanoscale, 2016, 8, 11924
    DOI: 10.1039/C6NR02151D

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