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Issue 14, 2016
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Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells

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Abstract

By combining super-resolution photoactivation localization microscopy with single particle tracking, we have visualized the endocytic process in the live-cell environment with nanoparticles (NPs) of different size and surface functionalization. This allowed us to analyze the dynamics of NPs interacting with cells with high spatial and temporal resolution. We identified two distinctly different types of pathways by which NPs are internalized via clathrin-coated pits (CCPs). Predominantly, NPs first bind to the membrane and, subsequently, CCPs form at this site. However, there are also instances where a NP diffuses on the membrane and utilizes a preformed CCP. Moreover, we have applied this new method to further explore the effects of size and surface functionalization on the NP dynamics on the plasma membrane and the ensuing endocytosis.

Graphical abstract: Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells

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Publication details

The article was received on 22 Feb 2016, accepted on 14 Mar 2016 and first published on 16 Mar 2016


Article type: Communication
DOI: 10.1039/C6NR01495J
Citation: Nanoscale, 2016,8, 7423-7429
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    Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells

    Y. Li, L. Shang and G. U. Nienhaus, Nanoscale, 2016, 8, 7423
    DOI: 10.1039/C6NR01495J

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