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Issue 2, 2016
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New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

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Abstract

Covering: 2010 to 2015

Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.

Graphical abstract: New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

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Publication details

The article was received on 02 Aug 2015 and first published on 09 Dec 2015


Article type: Highlight
DOI: 10.1039/C5NP00085H
Author version available: Download Author version (PDF)
Citation: Nat. Prod. Rep., 2016,33, 174-182
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    New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

    Y. Luo, B. Enghiad and H. Zhao, Nat. Prod. Rep., 2016, 33, 174
    DOI: 10.1039/C5NP00085H

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