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Issue 3, 2016
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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

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Abstract

Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.

Graphical abstract: Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

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Publication details

The article was received on 05 Aug 2016, accepted on 23 Aug 2016 and first published on 07 Sep 2016


Article type: Communication
DOI: 10.1039/C6ME00073H
Citation: Mol. Syst. Des. Eng., 2016,1, 273-277
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    Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

    B. E. Tam, K. Sung and H. D. Sikes, Mol. Syst. Des. Eng., 2016, 1, 273
    DOI: 10.1039/C6ME00073H

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