Issue 2, 2017

Ligand-responsive upregulation of 3′ CITE-mediated translation in a wheat germ cell-free expression system

Abstract

We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3′ cap-independent translation element (3′ CITE) with no major hybridization switches in a plant expression system (wheat germ extract).

Graphical abstract: Ligand-responsive upregulation of 3′ CITE-mediated translation in a wheat germ cell-free expression system

Supplementary files

Article information

Article type
Communication
Submitted
02 Nov 2016
Accepted
05 Dec 2016
First published
07 Dec 2016

Mol. BioSyst., 2017,13, 314-319

Ligand-responsive upregulation of 3′ CITE-mediated translation in a wheat germ cell-free expression system

A. Ogawa, Y. Murashige, J. Tabuchi and T. Omatsu, Mol. BioSyst., 2017, 13, 314 DOI: 10.1039/C6MB00748A

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