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Issue 10, 2016
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Biomolecular proteomics discloses ATP synthase as the main target of the natural glycoside deglucoruscin

Federica del Gaudio,abc  Carmen Festa,d  Matteo Mozzicafreddo,e  Michele Vasaturo,a  Agostino Casapullo,a  Simona De Marino,d  Raffaele Riccioa  and  Maria Chiara Monti*a 
Author affiliations

* Corresponding authors

a Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, Fisciano, Italy
E-mail: mcmonti@unisa.it
Fax: +39-089-969600
Tel: +39-089-969453

b PhD Program in Drug Discovery and Development, Università degli Studi di Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano (SA), Italy

c Farmaceutici Damor S.p.A, Via E. Scaglione 27, 80145 Naples, Italy

d Department of Pharmacy, University of Naples “Federico II”, Via D. Montesano, 49, I-80131 Naples, Italy

e School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy

Abstract

Extracts of Ruscus aculeatus are a rich source of bioactive steroidal glycosides, such as ruscogenins which are reported to act against chronic venous disorders. Nowadays, several preparations of its roots, commonly used in traditional medicine, are on the market as food supplements for health care and maintenance. Although spirostanol deglucoruscin is one of the main metabolites in these extracts, literature reports about its pharmacological profile are scarce. In this paper, a multi-disciplinary approach, based on chemical proteomics, molecular modelling and bio-organic assays, has been used to disclose the whole interactome of deglucoruscin and the F0–F1 ATP synthase complex has been found as its main target.

Graphical abstract: Biomolecular proteomics discloses ATP synthase as the main target of the natural glycoside deglucoruscin

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Publication details

The article was received on 10 Jun 2016, accepted on 25 Jul 2016 and first published on 25 Jul 2016


Article type: Paper
DOI: 10.1039/C6MB00460A
Citation: Mol. BioSyst., 2016,12, 3132-3138
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    Biomolecular proteomics discloses ATP synthase as the main target of the natural glycoside deglucoruscin

    F. del Gaudio, C. Festa, M. Mozzicafreddo, M. Vasaturo, A. Casapullo, S. De Marino, R. Riccio and M. C. Monti, Mol. BioSyst., 2016, 12, 3132
    DOI: 10.1039/C6MB00460A

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