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Issue 24, 2016
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Single-cell isolation by a modular single-cell pipette for RNA-sequencing

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Abstract

Single-cell transcriptome sequencing highly requires a convenient and reliable method to rapidly isolate a live cell into a specific container such as a PCR tube. Here, we report a modular single-cell pipette (mSCP) consisting of three modular components, a SCP-Tip, an air-displacement pipette (ADP), and ADP-Tips, that can be easily assembled, disassembled, and reassembled. By assembling the SCP-Tip containing a hydrodynamic trap, the mSCP can isolate single cells from 5–10 cells per μL of cell suspension. The mSCP is compatible with microscopic identification of captured single cells to finally achieve 100% single-cell isolation efficiency. The isolated live single cells are in submicroliter volumes and well suitable for single-cell PCR analysis and RNA-sequencing. The mSCP possesses merits of convenience, rapidness, and high efficiency, making it a powerful tool to isolate single cells for transcriptome analysis.

Graphical abstract: Single-cell isolation by a modular single-cell pipette for RNA-sequencing

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Publication details

The article was received on 04 Oct 2016, accepted on 02 Nov 2016 and first published on 02 Nov 2016


Article type: Paper
DOI: 10.1039/C6LC01241H
Citation: Lab Chip, 2016,16, 4742-4748
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    Single-cell isolation by a modular single-cell pipette for RNA-sequencing

    K. Zhang, M. Gao, Z. Chong, Y. Li, X. Han, R. Chen and L. Qin, Lab Chip, 2016, 16, 4742
    DOI: 10.1039/C6LC01241H

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