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Issue 19, 2016
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Microfluidic partition with in situ biofabricated semipermeable biopolymer membranes for static gradient generation

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Abstract

We report an in situ biofabrication strategy that conveniently partitions microfluidic networks into physically separated while chemically communicating microchannels with semipermeable biopolymer membranes, which enable the facile generation of static gradients for biomedical applications. The biofabrication of parallel biopolymer membranes was initiated with the dissipation of trapped air bubbles in parallel apertures in polydimethylsiloxane (PDMS) microfluidic devices, followed by tunable membrane growth with precise temporal and spatial control to the desired thickness. Static gradients were generated within minutes and well maintained over time by pure diffusion of molecules through the biofabricated semipermeable membranes. As an example application, the static gradient of alpha factor was generated to study the development of the “shmoo” morphology of yeast over time. The in situ biofabrication provides a simple approach to generate static gradients and an ideal platform for biological applications where flow-free static gradients are indispensable.

Graphical abstract: Microfluidic partition with in situ biofabricated semipermeable biopolymer membranes for static gradient generation

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Publication details

The article was received on 08 Jun 2016, accepted on 16 Aug 2016 and first published on 16 Aug 2016


Article type: Paper
DOI: 10.1039/C6LC00742B
Citation: Lab Chip, 2016,16, 3815-3823
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    Microfluidic partition with in situ biofabricated semipermeable biopolymer membranes for static gradient generation

    X. Luo, T. Vo, F. Jambi, P. Pham and J. S. Choy, Lab Chip, 2016, 16, 3815
    DOI: 10.1039/C6LC00742B

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