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Issue 12, 2016
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Human induced pluripotent stem cell-derived fiber-shaped cardiac tissue on a chip

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Abstract

We propose a method for the production of a fiber-shaped three-dimensional (3D) cellular construct of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) for the quantification of the contractile force. By culturing the cardiomyocytes in a patterned hydrogel structure with fixed edges, we succeeded in fabricating hiPS-CM fibers with aligned cardiomyocytes. The fiber generated contractile force along the fiber direction due to the hiPS-CM alignment, and we were able to measure its contractile force accurately. Furthermore, to demonstrate the drug reactivity of hiPS-CM fibers, the changes in the contractile frequency and force following treatment with isoproterenol and propranolol were observed. We believe that hiPS-CM fibers will be a useful tool for pharmacokinetic analyses during drug development.

Graphical abstract: Human induced pluripotent stem cell-derived fiber-shaped cardiac tissue on a chip

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Publication details

The article was received on 30 Mar 2016, accepted on 09 May 2016 and first published on 09 May 2016


Article type: Paper
DOI: 10.1039/C6LC00422A
Citation: Lab Chip, 2016,16, 2295-2301
  • Open access: Creative Commons BY-NC license
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    Human induced pluripotent stem cell-derived fiber-shaped cardiac tissue on a chip

    Y. Morimoto, S. Mori, F. Sakai and S. Takeuchi, Lab Chip, 2016, 16, 2295
    DOI: 10.1039/C6LC00422A

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