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Issue 13, 2016
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High accuracy particle analysis using sheathless microfluidic impedance cytometry

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Abstract

This paper describes a new design of microfluidic impedance cytometer enabling accurate characterization of particles without the need for focusing. The approach uses multiple pairs of electrodes to measure the transit time of particles through the device in two simultaneous different current measurements, a transverse (top to bottom) current and an oblique current. This gives a new metric that can be used to estimate the vertical position of the particle trajectory through the microchannel. This parameter effectively compensates for the non-uniform electric field in the channel that is an unavoidable consequence of the use of planar parallel facing electrodes. The new technique is explained and validated using numerical modelling. Impedance data for 5, 6 and 7 μm particles are collected and compared with simulations. The method gives excellent coefficient of variation in (electrical) radius of particles of 1% for a sheathless configuration.

Graphical abstract: High accuracy particle analysis using sheathless microfluidic impedance cytometry

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Publication details

The article was received on 10 Mar 2016, accepted on 20 May 2016 and first published on 20 May 2016


Article type: Paper
DOI: 10.1039/C6LC00339G
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2016,16, 2467-2473
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    High accuracy particle analysis using sheathless microfluidic impedance cytometry

    D. Spencer, F. Caselli, P. Bisegna and H. Morgan, Lab Chip, 2016, 16, 2467
    DOI: 10.1039/C6LC00339G

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