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Issue 8, 2016
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Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

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Abstract

Droplet-based microfluidics enables assays to be carried out at very high throughput (up to thousands of samples per second) and enables researchers to work with very limited material, such as primary cells, patient's biopsies or expensive reagents. An additional strength of the technology is the possibility to perform large-scale genotypic or phenotypic screens at the single-cell level. Here we critically review the latest developments in antibody screening, drug discovery and highly multiplexed genomic applications such as targeted genetic workflows, single-cell RNAseq and single-cell ChIPseq. Starting with a comprehensive introduction for non-experts, we pinpoint current limitations, analyze how they might be overcome and give an outlook on exciting future applications.

Graphical abstract: Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

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Publication details

The article was received on 23 Feb 2016, accepted on 18 Mar 2016 and first published on 18 Mar 2016


Article type: Critical Review
DOI: 10.1039/C6LC00249H
Citation: Lab Chip, 2016,16, 1314-1331
  • Open access: Creative Commons BY license
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    Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

    N. Shembekar, C. Chaipan, R. Utharala and C. A. Merten, Lab Chip, 2016, 16, 1314
    DOI: 10.1039/C6LC00249H

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