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Issue 1, 2016
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Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis

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Abstract

We describe the design, fabrication, and successful demonstration of a sample preparation module comprising bacteria cell capture and thermal lysis on-chip with potential applications in food sample pathogen analysis. Plasma nanotexturing of the polymeric substrate allows increase of the surface area of the chip and the antibody binding capacity. Three different anti-Salmonella antibodies were directly and covalently linked to plasma treated chips without any additional linker chemistry or other treatment. Then, the Ab-modified chips were tested for their capacity to bind bacteria in the concentration range of 102–108 cells per mL; the module exhibited 100% efficiency in Salmonella enterica serovar Typhimurium bacteria capture for cell suspensions below 105 cells per mL (104 cells injected with a 100 μL sample volume) and efficiency higher than 50% for 107 cells per mL. Moreover, thermal lysis achieved on-chip from as low as 10 captured cells was demonstrated and shown to compare well with off-chip lysis. Excellent selectivity (over 1 : 300) was obtained in a sample containing, in addition to S. Typhimurium and E. coli bacteria.

Graphical abstract: Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis

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Publication details

The article was received on 04 Oct 2015, accepted on 28 Oct 2015 and first published on 29 Oct 2015


Article type: Paper
DOI: 10.1039/C5LC01217A
Citation: Lab Chip, 2016,16, 120-131
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    Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis

    K. Tsougeni, G. Papadakis, M. Gianneli, A. Grammoustianou, V. Constantoudis, B. Dupuy, P. S. Petrou, S. E. Kakabakos, A. Tserepi, E. Gizeli and E. Gogolides, Lab Chip, 2016, 16, 120
    DOI: 10.1039/C5LC01217A

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