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Issue 5, 2016
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Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette

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Abstract

We demonstrate a standalone microfluidic chip that allows us to carry out commonly executed antibiotic susceptibility assays in an array of nanoliter droplets. We eliminated the need for automation in performing an exemplary complicated liquid handling assay on a chip. Operations on droplets are hard-wired into the microfluidic chip. The liquid handling protocol can be executed with a simple and commonly available source of flow such as an automatic manual pipette. The system passively prepares a series of dilutions of a chemical compound and mixes them with portions of the sample. The precision of metering, merging, mixing, and splitting of discrete portions of liquid samples is rooted in the passive capillary action in microfluidic traps and not in the precision of dosing with a pipette. We show an exemplary use of the device in the determination of the minimum inhibitory concentration (MIC) of ampicillin against E. coli ATCC 25922.

Graphical abstract: Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette

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Publication details

The article was received on 24 Sep 2015, accepted on 12 Jan 2016 and first published on 12 Jan 2016


Article type: Paper
DOI: 10.1039/C5LC01151E
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Citation: Lab Chip, 2016,16, 893-901
  • Open access: Creative Commons BY-NC license
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    Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette

    L. Derzsi, T. S. Kaminski and P. Garstecki, Lab Chip, 2016, 16, 893
    DOI: 10.1039/C5LC01151E

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