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Issue 3, 2016
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A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

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Abstract

Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.

Graphical abstract: A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

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Publication details

The article was received on 16 Sep 2015, accepted on 20 Nov 2015 and first published on 20 Nov 2015


Article type: Paper
DOI: 10.1039/C5LC01117E
Citation: Lab Chip, 2016,16, 489-496
  • Open access: Creative Commons BY-NC license
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    A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis

    Z. Zhao, Y. Yang, Y. Zeng and M. He, Lab Chip, 2016, 16, 489
    DOI: 10.1039/C5LC01117E

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