Jump to main content
Jump to site search

Issue 4, 2016
Previous Article Next Article

Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software: a practical guide

Author affiliations

Abstract

The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.

Graphical abstract: Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software: a practical guide

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Nov 2015, accepted on 27 Jan 2016 and first published on 29 Jan 2016


Article type: Tutorial Review
DOI: 10.1039/C5JA00451A
Author version available: Download Author version (PDF)
Citation: J. Anal. At. Spectrom., 2016,31, 832-840
  •   Request permissions

    Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software: a practical guide

    G. de S. Pessôa, J. L. Capelo-Martínez, F. Fdez-Riverola, H. López-Fernández, D. Glez-Peña, M. Reboiro-Jato and M. A. Z. Arruda, J. Anal. At. Spectrom., 2016, 31, 832
    DOI: 10.1039/C5JA00451A

Search articles by author

Spotlight

Advertisements