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Issue 5, 2016
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Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy

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Abstract

Chemical imaging of single cells at the molecular level is important in capturing biological dynamics. Single cell correlative imaging is realized between super-resolution microscopy, namely, structured illumination microscopy (SIM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a multimodal microreactor (i.e., System for Analysis at the Liquid Vacuum Interface, SALVI). SIM characterized cells and guided subsequent ToF-SIMS analysis. Lipid fragments were identified in the cell membrane via dynamic ToF-SIMS depth profiling. Positive SIMS spectra show intracellular potassium and sodium ion transport due to exposure to nanoparticles. Spectral principal component analysis elucidates differences in chemical composition among healthy alveolar epithelial mouse lung C10 cells, cells that uptake zinc oxide nanoparticles, and various wet and dry control samples. The observation of Zn+ gives the first direct evidence of ZnO NP uptake and dissolution by the cell membrane. Our results provide submicron chemical mapping for investigating cell dynamics at the molecular level.

Graphical abstract: Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy

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Publication details

The article was received on 01 Dec 2015, accepted on 18 Mar 2016 and first published on 22 Mar 2016


Article type: Technical Innovation
DOI: 10.1039/C5IB00308C
Citation: Integr. Biol., 2016,8, 635-644
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    Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy

    X. Hua, C. Szymanski, Z. Wang, Y. Zhou, X. Ma, J. Yu, J. Evans, G. Orr, S. Liu, Z. Zhu and X. Yu, Integr. Biol., 2016, 8, 635
    DOI: 10.1039/C5IB00308C

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