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Issue 10, 2016
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Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose

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Abstract

Conversion of biomass using biocatalysis is likely to become a technology that contributes significantly to the future production of chemical building blocks, materials and transport fuels. Here the synthesis of a value-added chemical from L-arabinose, a major component of the carbohydrates in sugar beet pulp (SBP), in a concise and sustainable manner has been investigated. Biocatalytic conversions using transketolase variants have been developed for the efficient, scalable synthesis of a rare naturally occurring ketoheptose, L-gluco-heptulose, from L-arabinose. New active E. coli TK mutants that readily accept L-arabinose were identified using a versatile colorimetric screening assay and the reaction was performed on a preparative scale.

Graphical abstract: Transketolase catalysed upgrading of l-arabinose: the one-step stereoselective synthesis of l-gluco-heptulose

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Publication details

The article was received on 05 Nov 2015, accepted on 16 Feb 2016 and first published on 16 Feb 2016


Article type: Paper
DOI: 10.1039/C5GC02660A
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Citation: Green Chem., 2016,18, 3158-3165
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    Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose

    F. Subrizi, M. Cárdenas-Fernández, G. J. Lye, J. M. Ward, P. A. Dalby, T. D. Sheppard and H. C. Hailes, Green Chem., 2016, 18, 3158
    DOI: 10.1039/C5GC02660A

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