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Volume 187, 2016
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Advancements in quantum cascade laser-based infrared microscopy of aqueous media

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Abstract

The large mid-infrared absorption coefficient of water frequently hampers the rapid, label-free infrared microscopy of biological objects in their natural aqueous environment. However, the high spectral power density of quantum cascade lasers is shifting this limitation such that mid-infrared absorbance images can be acquired in situ within signal-to-noise ratios of up to 100. Even at sample thicknesses well above 50 μm, signal-to-noise ratios above 10 are readily achieved. The quantum cascade laser-based microspectroscopy of aqueous media is exemplified by imaging an aqueous yeast solution and quantifying glucose consumption, ethanol generation as well as the production of carbon dioxide gas during fermentation.

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Publication details

The article was received on 17 Nov 2015, accepted on 14 Dec 2015 and first published on 14 Dec 2015


Article type: Paper
DOI: 10.1039/C5FD00177C
Citation: Faraday Discuss., 2016,187, 119-134
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    Advancements in quantum cascade laser-based infrared microscopy of aqueous media

    K. Haase, N. Kröger-Lui, A. Pucci, A. Schönhals and W. Petrich, Faraday Discuss., 2016, 187, 119
    DOI: 10.1039/C5FD00177C

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