Jump to main content
Jump to site search

Issue 3, 2016
Previous Article Next Article

The effects of rosette nanotubes with different functionalizations on channel catfish (Ictalurus punctatus) lymphocyte viability and receptor function

Author affiliations

Abstract

The effects of self-assembled rosette nanotubes (RNTs) with different surface functionalizations (K-, TBL-, RGDSK1/TBL9-) on fish lymphocyte viability and effector function were examined using channel catfish (Ictalurus punctatus) as a model. Surface functionalization was an important determinant of nanotoxicity. Lysine-functionalized RNTs (K-RNTs) consistently caused the greatest decline in lymphocyte viability while aminobutyl-functionalized RNTs (TBL-RNTs) had the least effect on lymphocyte viability. This trend was conserved across the multiple cell lines examined (both B- and T-cells). However, the absolute change in viability was distinct for each type of lymphocyte studied. Following RNT exposure, the two channel catfish B-cell lines tested, 3B11 and 1G8, had similar toxicity profiles for each of the RNT functionalizations examined. This was in contrast to the T-cell line tested, 28S.3, which had more viable cells remaining in culture post RNT exposure and suggests differences in toxicity based on the lymphocyte examined (B- versus T-cells). Lastly, exposure of cells to K-, TBL- or Arg-Gly-Asp-Ser-Lys peptide-functionalized RNTs (RGDSK-RNTs), significantly reduced the ability of cells to phagocytose through the channel catfish immune receptor, leukocyte immune-type receptor (IpLITR). Sub-lethal levels of RNT exposure affected the ability of immune cells to elicit this effector response in vitro and was concentration- and functionalization-dependent. Together, these data demonstrate that distinct classes of fish lymphocytes respond differentially to RNT exposure and that RNT functionalization impacts both lymphocyte viability and effector functions.

Graphical abstract: The effects of rosette nanotubes with different functionalizations on channel catfish (Ictalurus punctatus) lymphocyte viability and receptor function

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Jan 2016, accepted on 26 Apr 2016 and first published on 27 Apr 2016


Article type: Paper
DOI: 10.1039/C6EN00024J
Citation: Environ. Sci.: Nano, 2016,3, 578-592
  •   Request permissions

    The effects of rosette nanotubes with different functionalizations on channel catfish (Ictalurus punctatus) lymphocyte viability and receptor function

    J. D. Ede, V. A. Ortega, D. Boyle, R. L. Beingessner, U. D. Hemraz, H. Fenniri, J. L. Stafford and G. G. Goss, Environ. Sci.: Nano, 2016, 3, 578
    DOI: 10.1039/C6EN00024J

Search articles by author

Spotlight

Advertisements