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Issue 24, 2016
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CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering

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Abstract

The CRISPR–Cas9 system has revolutionized the process of making changes to the DNA sequence of organisms. Relying on a simplistic model of RNA guided DNA binding and cleavage, this molecular toolbox has found application in nearly every branch of biological sciences. The story of CRISPR–Cas9 is one of discovery and development where a component of bacterial adaptive immunity has been harnessed to address important biological questions using significant inputs from physicochemical structure–function studies. In this review, we trace the evolution of CRISPR–Cas9 from its predecessor genome editing tools and document its current status with an emphasis on chemical biology aspects of modulating its activity to generate a potent tool for gene therapy applications.

Graphical abstract: CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering

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Publication details

The article was received on 10 Mar 2016, published on 14 Sep 2016 and first published online on 14 Sep 2016


Article type: Tutorial Review
DOI: 10.1039/C6CS00197A
Citation: Chem. Soc. Rev., 2016,45, 6666-6684
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    CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering

    A. Singh, D. Chakraborty and S. Maiti, Chem. Soc. Rev., 2016, 45, 6666
    DOI: 10.1039/C6CS00197A

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