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Issue 12, 2016
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Adsorption according to the Langmuir–Freundlich model is the detection mechanism of the antigen p53 for early diagnosis of cancer

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Abstract

Biosensors for early detection of cancer biomarkers normally depend on specific interactions between such biomarkers and immobilized biomolecules in the sensing units. Though these interactions are expected to yield specific, irreversible adsorption, the underlying mechanism appears not to have been studied in detail. In this paper, we show that adsorption explained with the Langmuir–Freundlich model is responsible for detection of the antigen p53 associated with various types of cancers. Irreversible adsorption was proven between anti-p53 antibodies immobilized on the biosensors and the antigen p53, with the adequacy of the Langmuir–Freundlich model being confirmed with three independent experimental methods, viz. polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), nanogravimetry using a quartz crystal microbalance and electrochemical impedance spectroscopy. The method based on this irreversible adsorption was sufficiently sensitive (limit of detection of 1.4 pg mL−1) for early diagnosis of Hodgkin lymphoma, pancreatic and colon carcinomas, and bladder, ovarian and lung cancers, and could distinguish between MCF7 cells containing the antigen p53 from Saos-2 cells that do not contain it.

Graphical abstract: Adsorption according to the Langmuir–Freundlich model is the detection mechanism of the antigen p53 for early diagnosis of cancer

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Publication details

The article was received on 19 Nov 2015, accepted on 09 Feb 2016 and first published on 11 Feb 2016


Article type: Paper
DOI: 10.1039/C5CP07121F
Citation: Phys. Chem. Chem. Phys., 2016,18, 8412-8418
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    Adsorption according to the Langmuir–Freundlich model is the detection mechanism of the antigen p53 for early diagnosis of cancer

    J. C. Soares, A. C. Soares, P. A. R. Pereira, V. D. C. Rodrigues, F. M. Shimizu, M. E. Melendez, C. Scapulatempo Neto, A. L. Carvalho, F. L. Leite, S. A. S. Machado and O. N. Oliveira, Phys. Chem. Chem. Phys., 2016, 18, 8412
    DOI: 10.1039/C5CP07121F

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