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Issue 75, 2016
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A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

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Abstract

Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.

Graphical abstract: A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

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Publication details

The article was received on 21 Jul 2016, accepted on 19 Aug 2016 and first published on 19 Aug 2016


Article type: Communication
DOI: 10.1039/C6CC05990B
Citation: Chem. Commun., 2016,52, 11239-11242
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    A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

    X. Zhu, P. Shieh, M. Su, C. R. Bertozzi and W. Zhang, Chem. Commun., 2016, 52, 11239
    DOI: 10.1039/C6CC05990B

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