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Issue 21, 2016
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Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration

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Abstract

NMR characterization of many proteins is limited by low expression, hurdles for deuteration, and poor sample homogeneity. We introduce a set of high-dimensionality proton-detected experiments developed for unambiguous resonance assignments of such proteins, which we could successfully apply to a 1 mg amount of non-deuterated Tau paired helical filaments.

Graphical abstract: Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration

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Publication details

The article was received on 04 Nov 2015, accepted on 19 Jan 2016 and first published on 19 Jan 2016


Article type: Communication
DOI: 10.1039/C5CC09160H
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Citation: Chem. Commun., 2016,52, 4002-4005
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    Backbone assignment for minimal protein amounts of low structural homogeneity in the absence of deuteration

    S. Xiang, J. Biernat, E. Mandelkow, S. Becker and R. Linser, Chem. Commun., 2016, 52, 4002
    DOI: 10.1039/C5CC09160H

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