Issue 5, 2016

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Abstract

A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.

Graphical abstract: Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Supplementary files

Article information

Article type
Communication
Submitted
04 Nov 2015
Accepted
13 Nov 2015
First published
13 Nov 2015

Chem. Commun., 2016,52, 954-957

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

L. Tan, Y. Liu, Q. Yang, X. Li, X. Wu, B. Gong, Y. Shen and Z. Shao, Chem. Commun., 2016, 52, 954 DOI: 10.1039/C5CC09131D

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