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Issue 5, 2016
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Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

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Abstract

A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.

Graphical abstract: Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

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Publication details

The article was received on 04 Nov 2015, accepted on 13 Nov 2015 and first published on 13 Nov 2015


Article type: Communication
DOI: 10.1039/C5CC09131D
Citation: Chem. Commun., 2016,52, 954-957
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    Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

    L. Tan, Y. Liu, Q. Yang, X. Li, X. Wu, B. Gong, Y. Shen and Z. Shao, Chem. Commun., 2016, 52, 954
    DOI: 10.1039/C5CC09131D

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