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Issue 11, 2016
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PEG-based hydrogels prepared by catalyst-free thiol–yne addition and their post-antibacterial modification

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Abstract

PEG-based hydrogels possess tissue-like mechanical elasticity, solute permeability, cytocompatibility and biocompatibility. In this work, PEG-based hydrogels were prepared via nucleophilic thiol–yne addition between a 4-arm PEG functionalized with thiols (PEG10k-4-SH) and an electron-deficient alkyne (PEG10k-4-PP). The as-fabricated hydrogels still possess residual functionalities, enabling a second nucleophilic thiol–yne addition on the gel matrix. A thiol-containing fluorescent dye was conjugated with the electron-deficient alkyne appended hydrogels. A thiol-containing antimicrobial peptide (AMP-SH) was also embedded into the gel matrix via nucleophilic thiol–yne addition. The inhibition of bacterial growth in suspensions and contaminated substrate surfaces by the AMP-embedded hydrogels was studied. The cytotoxicity of unmodified and AMP-embedded PEG-based hydrogels against 3T3 fibroblasts was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) viability assay.

Graphical abstract: PEG-based hydrogels prepared by catalyst-free thiol–yne addition and their post-antibacterial modification

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Publication details

The article was received on 12 Jun 2016, accepted on 16 Sep 2016 and first published on 29 Sep 2016


Article type: Paper
DOI: 10.1039/C6BM00395H
Citation: Biomater. Sci., 2016,4, 1663-1672
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    PEG-based hydrogels prepared by catalyst-free thiol–yne addition and their post-antibacterial modification

    X. Y. Cai, J. Z. Li, N. N. Li, J. C. Chen, E. Kang and L. Q. Xu, Biomater. Sci., 2016, 4, 1663
    DOI: 10.1039/C6BM00395H

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