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Issue 13, 2016
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Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

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Abstract

Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium–silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.

Graphical abstract: Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

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Publication details

The article was received on 15 Dec 2015, accepted on 19 Jan 2016 and first published on 19 Jan 2016


Article type: Communication
DOI: 10.1039/C5AN02573G
Citation: Analyst, 2016,141, 3982-3984
  • Open access: Creative Commons BY license
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    Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

    G. Faccio, M. B. Bannwarth, C. Schulenburg, V. Steffen, D. Jankowska, M. Pohl, R. M. Rossi, K. Maniura-Weber, L. F. Boesel and M. Richter, Analyst, 2016, 141, 3982
    DOI: 10.1039/C5AN02573G

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