Jump to main content
Jump to site search

Issue 20, 2016
Previous Article Next Article

Exploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy

Author affiliations

Abstract

The aim of this research was to find the optimal Raman excitation wavelength to attain the largest possible sensitivity in deep Raman spectroscopy of breast tissue. This involved careful consideration of factors such as tissue absorption, scattering, fluorescence and instrument response function. The study examined the tissue absorption profile combined with Raman scattering and detection sensitivity at seven different, laser excitation wavelengths in the near infrared region of the spectrum. Several key scenarios in regards to the sample position within the tissue were examined. The highest Raman band visibility over the background ratio in respect to biological tissue provides the necessary information for determining the optimum laser excitation wavelength for deep tissue analysis using transmission Raman spectroscopy, including detection of breast calcifications. For thick tissues with a mix of protein and fat, such as breast tissue, 790–810 nm is concluded to be the optimum excitation wavelength for deep Raman measurements.

Graphical abstract: Exploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Feb 2016, accepted on 19 Jul 2016 and first published on 27 Jul 2016


Article type: Paper
DOI: 10.1039/C6AN00490C
Citation: Analyst, 2016,141, 5738-5746
  • Open access: Creative Commons BY license
  •   Request permissions

    Exploring the effect of laser excitation wavelength on signal recovery with deep tissue transmission Raman spectroscopy

    A. Ghita, P. Matousek and N. Stone, Analyst, 2016, 141, 5738
    DOI: 10.1039/C6AN00490C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements