Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

Yuanyuan Wang; Zhaopeng Yu; Zhen Zhang; Rui Ren; Shusheng Zhang

doi:10.1039/C6AN00160B

Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

Yuanyuan Wang,^a Zhaopeng Yu,^b Zhen Zhang,*^b Rui Ren^a^b and Shusheng Zhang^b

Author affiliations

* Corresponding authors

^a Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China

^b Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China
E-mail: zhangzhen19801981@126.com
Fax: +86-539-8766867
Tel: +86-539-8766867

Abstract

Orderly nucleic acid aggregates (ONAAs) self-assembled on mesoporous silica nanoparticles (MSNs) with positively charged aminopropyl groups (PC) were firstly developed. Interestingly, a novel electrostatic DNA self-assembly could realize hybridization chain reaction (HCR) on the surface of PCMSNs in single cells. Significantly, a non-destructive amplification strategy based on ONAAs-PCMSNs was successfully developed for miRNA detection and in situ imaging by the prominent and agminated fluorescence-bright spots in living cells.

Supplementary files

Publication details

The article was received on 23 Jan 2016, accepted on 29 Mar 2016 and first published on 29 Mar 2016

Article type: Communication

DOI: 10.1039/C6AN00160B

Citation: Analyst, 2016,141, 2861-2864

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Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

Y. Wang, Z. Yu, Z. Zhang, R. Ren and S. Zhang, Analyst, 2016, 141, 2861
DOI: 10.1039/C6AN00160B
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Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

Abstract

Supplementary files

Publication details

Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

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