Jump to main content
Jump to site search

Issue 12, 2016
Previous Article Next Article

Combining random forest and 2D correlation analysis to identify serum spectral signatures for neuro-oncology

Author affiliations

Abstract

Fourier transform infrared (FTIR) spectroscopy has long been established as an analytical technique for the measurement of vibrational modes of molecular systems. More recently, FTIR has been used for the analysis of biofluids with the aim of becoming a tool to aid diagnosis. For the clinician, this represents a convenient, fast, non-subjective option for the study of biofluids and the diagnosis of disease states. The patient also benefits from this method, as the procedure for the collection of serum is much less invasive and stressful than traditional biopsy. This is especially true of patients in whom brain cancer is suspected. A brain biopsy is very unpleasant for the patient, potentially dangerous and can occasionally be inconclusive. We therefore present a method for the diagnosis of brain cancer from serum samples using FTIR and machine learning techniques. The scope of the study involved 433 patients from whom were collected 9 spectra each in the range 600–4000 cm−1. To begin the development of the novel method, various pre-processing steps were investigated and ranked in terms of final accuracy of the diagnosis. Random forest machine learning was utilised as a classifier to separate patients into cancer or non-cancer categories based upon the intensities of wavenumbers present in their spectra. Generalised 2D correlational analysis was then employed to further augment the machine learning, and also to establish spectral features important for the distinction between cancer and non-cancer serum samples. Using these methods, sensitivities of up to 92.8% and specificities of up to 91.5% were possible. Furthermore, ratiometrics were also investigated in order to establish any correlations present in the dataset. We show a rapid, computationally light, accurate, statistically robust methodology for the identification of spectral features present in differing disease states. With current advances in IR technology, such as the development of rapid discrete frequency collection, this approach is of importance to enable future clinical translation and enables IR to achieve its potential.

Graphical abstract: Combining random forest and 2D correlation analysis to identify serum spectral signatures for neuro-oncology

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Nov 2015, accepted on 19 Jan 2016 and first published on 19 Jan 2016


Article type: Paper
DOI: 10.1039/C5AN02452H
Citation: Analyst, 2016,141, 3668-3678
  •   Request permissions

    Combining random forest and 2D correlation analysis to identify serum spectral signatures for neuro-oncology

    B. R. Smith, K. M. Ashton, A. Brodbelt, T. Dawson, M. D. Jenkinson, N. T. Hunt, D. S. Palmer and M. J. Baker, Analyst, 2016, 141, 3668
    DOI: 10.1039/C5AN02452H

Search articles by author

Spotlight

Advertisements