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Issue 44, 2015
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Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

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Abstract

Non-invasive measurement of the membrane tension of free-standing black lipid membranes (BLMs), with sensitivity on the order of μN m−1, was achieved using laser-induced surface deformation (LISD) spectroscopy. A BLM was vertically formed via the folding method and aqueous phases with different refractive indices were added on each side in order to induce radiation pressure by a laser beam. The dynamic response of the deformed BLMs was measured under periodic intensity modulation and their tensions could be estimated. The dependence of membrane tension on the cholesterol concentration of BLMs composed of phosphatidylcholine and phosphatidylethanolamine was investigated, with the membrane tension increasing from 1.3 μN m−1 to 68.1 μN m−1 when the cholesterol concentration increased from zero to 33%. These tension values are much smaller than some of those previously reported, because this method does not suppress membrane fluctuation unlike other conventional methods. Our LISD system can be a promising tool for the measurement of membrane tension in BLMs.

Graphical abstract: Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

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Publication details

The article was received on 23 May 2015, accepted on 03 Sep 2015 and first published on 07 Sep 2015


Article type: Paper
DOI: 10.1039/C5SM01264C
Author version available: Download Author version (PDF)
Citation: Soft Matter, 2015,11, 8641-8647
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    Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

    T. Takei, T. Yaguchi, T. Fujii, T. Nomoto, T. Toyota and M. Fujinami, Soft Matter, 2015, 11, 8641
    DOI: 10.1039/C5SM01264C

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