Jump to main content
Jump to site search

Issue 3, 2015
Previous Article Next Article

Photostick: a method for selective isolation of target cells from culture

Author affiliations

Abstract

Sorting of target cells from a heterogeneous pool is technically difficult when the selection criterion is complex, e.g. a dynamic response, a morphological feature, or a combination of multiple parameters. At present, mammalian cell selections are typically performed either via static fluorescence (e.g. fluorescence activated cell sorter), via survival (e.g. antibiotic resistance), or via serial operations (flow cytometry, laser capture microdissection). Here we present a simple protocol for selecting cells based on any static or dynamic property that can be identified by video microscopy and image processing. The “photostick” technique uses a cell-impermeant photochemical crosslinker and digital micromirror array-based patterned illumination to immobilize selected cells on the culture dish. Other cells are washed away with mild protease treatment. The crosslinker also labels the selected cells with a fluorescent dye and a biotin for later identification. The photostick protocol preserves cell viability, permits genetic profiling of selected cells, and can be performed with complex functional selection criteria such as neuronal firing patterns.

Graphical abstract: Photostick: a method for selective isolation of target cells from culture

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Nov 2014, accepted on 07 Jan 2015 and first published on 21 Jan 2015


Article type: Edge Article
DOI: 10.1039/C4SC03676J
Citation: Chem. Sci., 2015,6, 1701-1705
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Photostick: a method for selective isolation of target cells from culture

    M. Chien, C. A. Werley, S. L. Farhi and A. E. Cohen, Chem. Sci., 2015, 6, 1701
    DOI: 10.1039/C4SC03676J

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements