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Issue 9, 2015
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Selective glycoprotein detection through covalent templating and allosteric click-imprinting

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Abstract

Many glycoproteins are intimately linked to the onset and progression of numerous heritable or acquired diseases of humans, including cancer. Indeed the recognition of specific glycoproteins remains a significant challenge in analytical method and diagnostic development. Herein, a hierarchical bottom-up route exploiting reversible covalent interactions with boronic acids and so-called click chemistry for the fabrication of glycoprotein selective surfaces that surmount current antibody constraints is described. The self-assembled and imprinted surfaces, containing specific glycoprotein molecular recognition nanocavities, confer high binding affinities, nanomolar sensitivity, exceptional glycoprotein specificity and selectivity with as high as 30 fold selectivity for prostate specific antigen (PSA) over other glycoproteins. This synthetic, robust and highly selective recognition platform can be used in complex biological media and be recycled multiple times with no performance decrement.

Graphical abstract: Selective glycoprotein detection through covalent templating and allosteric click-imprinting

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Publication details

The article was received on 07 Jun 2015, accepted on 15 Jun 2015 and first published on 17 Jun 2015


Article type: Edge Article
DOI: 10.1039/C5SC02031J
Citation: Chem. Sci., 2015,6, 5114-5119
  • Open access: Creative Commons BY license
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    Selective glycoprotein detection through covalent templating and allosteric click-imprinting

    A. Stephenson-Brown, A. L. Acton, J. A. Preece, J. S. Fossey and P. M. Mendes, Chem. Sci., 2015, 6, 5114
    DOI: 10.1039/C5SC02031J

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