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Issue 8, 2015
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Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

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Abstract

Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.

Graphical abstract: Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

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Publication details

The article was received on 08 Apr 2015, accepted on 08 Jun 2015 and first published on 08 Jun 2015


Article type: Edge Article
DOI: 10.1039/C5SC01258A
Citation: Chem. Sci., 2015,6, 4946-4953
  • Open access: Creative Commons BY license
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    Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

    T. Aslam, A. Miele, S. V. Chankeshwara, A. Megia-Fernandez, C. Michels, A. R. Akram, N. McDonald, N. Hirani, C. Haslett, M. Bradley and K. Dhaliwal, Chem. Sci., 2015, 6, 4946
    DOI: 10.1039/C5SC01258A

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