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Issue 6, 2015
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A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity

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Abstract

A peptide has been designed so that its chelating affinity for one type of metal ion regulates its affinity for a second, different type of metal ion. The prochelator peptide (PCP), which is a fusion of motifs evocative of calcium loops and zinc fingers, forms a 1 : 2 Zn : peptide complex at pH 7.4 that increases its affinity for Zn2+ ∼3-fold in the presence of Tb3+ (log β2 from 13.8 to 14.3), while the 1 : 1 luminescent complex with Tb3+ is brighter, longer lived, and 20-fold tighter in the presence of Zn2+ (log K from 6.2 to 7.5). This unique example of cooperative, heterometallic allostery in a biologically compatible construct suggests the possibility of designing conditionally active metal-binding agents that could respond to dynamic changes in cellular metal status.

Graphical abstract: A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity

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Publication details

The article was received on 16 Feb 2015, accepted on 22 Apr 2015 and first published on 05 May 2015


Article type: Edge Article
DOI: 10.1039/C5SC00602C
Citation: Chem. Sci., 2015,6, 3606-3610
  • Open access: Creative Commons BY-NC license
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    A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity

    B. Alies, J. D. Wiener and K. J. Franz, Chem. Sci., 2015, 6, 3606
    DOI: 10.1039/C5SC00602C

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

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