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Issue 8, 2015
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Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

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Abstract

The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.

Graphical abstract: Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

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Publication details

The article was received on 30 Nov 2014, accepted on 01 Jun 2015 and first published on 01 Jun 2015


Article type: Edge Article
DOI: 10.1039/C4SC03707C
Citation: Chem. Sci., 2015,6, 4837-4845
  • Open access: Creative Commons BY license
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    Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

    Z. Song, W. Bakeer, J. W. Marshall, A. A. Yakasai, R. M. Khalid, J. Collemare, E. Skellam, D. Tharreau, M. Lebrun, C. M. Lazarus, A. M. Bailey, T. J. Simpson and R. J. Cox, Chem. Sci., 2015, 6, 4837
    DOI: 10.1039/C4SC03707C

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